Crystallization and preliminary X-ray diffraction analysis of the wild-type haloalkane dehalogenase DhaA and its variant DhaA13 complexed with different ligands

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Crystallization and preliminary X-ray diffraction analysis of the wild-type haloalkane dehalogenase DhaA and its variant DhaA13 complexed with different ligands.

Haloalkane dehalogenases make up an important class of hydrolytic enzymes which catalyse the cleavage of carbon-halogen bonds in halogenated aliphatic compounds. There is growing interest in these enzymes owing to their potential use in environmental and industrial applications. The haloalkane dehalogenase DhaA from Rhodococcus rhodochrous NCIMB 13064 can slowly detoxify the industrial pollutan...

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Crystallization and preliminary X-ray diffraction

The amyloidogenic Leu55Pro variant of transthyretin has been expressed, purified and crystallized in space group C2. The cell constants are a--149.99, b = 78.74, c = 98.95A, = 100.5 ° and the crystals diffract to 2.7,~ resolution. There are eight monomers in the asymmetric unit giving a V M = 2.6,~,3 Da -w and 53% solvent content. In the wild-type protein, the crystals are orthorhombic with two...

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Crystallization and preliminary x-ray diffraction analysis of three mastoparans.

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Crystallization and preliminary X-ray diffraction analysis of the protease from Southampton norovirus complexed with a Michael acceptor inhibitor.

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ژورنال

عنوان ژورنال: Acta Crystallographica Section F Structural Biology and Crystallization Communications

سال: 2011

ISSN: 1744-3091

DOI: 10.1107/s1744309110051286